Enhanced relativistic-electron beam collimation using two consecutive laser pulses.

The double laser pulse approach to relativistic electron beam (REB) collimation in solid targets has been investigated at the LULI-ELFIE facility. In this scheme two collinear laser pulses are focused onto a solid target with a given intensity ratio and time delay to generate REBs. The magnetic field generated by the first laser-driven REB is used to guide the REB generated by a second delayed laser pulse. We show how electron beam collimation can be controlled by properly adjusting the ratio of focus size and the delay time between the two pulses. We found that the maximum of electron beam collimation is clearly dependent on the laser focal spot size ratio and related to the magnetic field dynamics. Cu-Kα and CTR imaging diagnostics were implemented to evaluate the collimation effects on the respectively low energy (≤100 keV) and high energy (≥MeV) components of the REB.

Atomic layer deposition-derived ultra-low-density composite bulk materials with deterministic density and composition.

A universal approach for on-demand development of monolithic metal oxide composite bulk materials with air-like densities (<5 mg/cm(3)) is reported. The materials are fabricated by atomic layer deposition of titania (TiO2) or zinc oxide (ZnO) using the nanoscale architecture of 1 mg/cm(3) SiO2 aerogels formed by self-organization as a blueprint. This approach provides deterministic control over density and composition without affecting the nanoscale architecture of the composite material that is otherwise very difficult to achieve. We found that these materials provide laser-to-X-ray conversion efficiencies of up to 5.3%, which is the highest conversion efficiency yet obtained from any foam-based target, thus opening the door to a new generation of highly efficient laser-induced nanosecond scale multi-keV X-ray sources.

Automated annotation removal in agar plates.

Agar plates are widely used in the biomedical field as a medium in which to artificially grow bacteria, algae or fungi. Agar plates (Petri dishes) are used routinely in microbiology laboratories in order to identify the type of micro-organism responsible for infections. Such diagnoses are based on counting the number and type of bacterial colonies growing in the Petri dish. The count of bacterial colonies is a time consuming task prone to human error, so interest in automated counting systems has increased in the recent years. One of the difficulties of automatizing the counting process is the presence of markers and annotations made in the lower part of the agar plate. Efficient removal of such markers can increase the accuracy of the bacterial counting system. This article introduces a fast method for detection, segmentation and removal of annotations in agar plates that improves the results of existing bacterial colony counting algorithms.

Functional simulation of the cochlea for implant optimization.

Cochlear implantation is a surgical technique which aims to restore hearing in people with deep hearing loss. However, outcomes of the surgery still exhibit a large variability between patients. Among the factors that contribute to variability the most important are morphological differences in anatomical structures between patients and incorrect implant placements. In order to address these issues, it would be desirable to have a functional model of the cochlea which incorporates inter-patients variability and simulate electrode placement. To this end, we present a finite element model which captures the interaction between the cochlear partition, modeled as an elastic solid with finite deformation, and the perilymph fluid, modeled as a compressible, viscous fluid. Numerical results show that the membrane responds to changes in the stimulation frequencies.

Meningitis pneumocócica precoz en neonato sano a término / Early-onset pneumococcal meningitis in a healthy full-term newborn

La sepsis neonatal se clasifica tradicionalmente, en precoz y tardía,en función del momento de la aparición de la sintomatología. Laprincipal finalidad de esta subdivisión es poder deducir cuáles sonlos agentes etiológicos más probables en cada caso y poder establecerel tratamiento etiológico más adecuado. Teniendo encuenta las implicaciones terapéuticas de estas definiciones, podríaser mejor hablar de sepsis vertical y sepsis horizontal, sin referenciasa límites tan estrictos como el tiempo. Se conoce sobradamenteque a partir de las 24 horas de vida las infecciones transmitidasverticalmente se pueden solapar con las de transmisiónhorizontal. Nuestro caso representa una evidencia puntual quesuscita cuestiones pendientes de resolver(AU)

Neonatal sepsis is classified in early-onset and late-onset sepsis, based on the timing of beginning of symptoms. The main purpose of this classification is to anticipate the most probable causative agents and administer the most suitable antibiotic treatment. Considering the therapeutic implications of those definitions, it would be probably most accurate to refer to sepsis of vertical and horizontal transmission, respectively, without time-references. It is known that after 24 hours from birth, both vertical and horizontal neonatal sepsis may occur. The case under discussion brings up unique scenarios of difficult classification(AU)

Abdominal distention results from caudo-ventral redistribution of contents.

BACKGROUND & AIMS: Abdominal bloating is a frequent symptom in various categories of patients; however, its origin is unclear. Our aim was to establish the mechanisms of abdominal bloating. METHODS: The study evaluated 56 patients whose predominant symptom was abdominal bloating. Of these, 47 (44 female and 3 male; aged 19-74 years) were diagnosed with functional intestinal disorder by Rome II criteria and 9 (7 female and 2 male; aged 18-64 years) were diagnosed with intestinal dysmotility by gastrointestinal manometry. Computed tomographic scans were obtained before (basal level) and during a severe bloating episode. Control scans were also obtained from 12 healthy subjects (11 female and 1 male; aged 19-62 years). Morpho-volumetric differences between basal and severe bloating scans were measured using an original computer analysis program. RESULTS: During severe bloating, patients with dysmotility exhibited anterior wall protrusion (23 +/- 4 mm; P < .001 vs basal) associated with a marked increase in total abdominal volume (1.4 +/- 0.3 L; P = .002 vs basal) and with cephalic displacement of the diaphragm. By contrast, in patients with functional intestinal disorder, total abdominal volume barely increased (0.3 +/- 0.1 L; P < .001 vs dysmotility); in these patients, abdominal distention (14 +/- 2 mm anterior wall protrusion; P < .001 vs basal) was related to diaphragmatic descent (-12 +/- 3 mm; R = -0.62; P < .001). CONCLUSIONS: Abdominal distention might be caused by an increase in intra-abdominal volume or abdomino-phrenic displacement and ventro-caudal redistribution of contents.

Surgical planning and patient-specific biomechanical simulation for tracheal endoprostheses interventions.

We have developed a system for computer-assisted surgical planning of tracheal surgeries. The system allows to plan the intervention based on CT images of the patient, and includes a virtual database of commercially available prostheses. Automatic segmentation of the trachea and apparent pathological structures is obtained using a modified region growing algorithm. A method for automatic adaptation of a finite element mesh allows to build a patient-specific biomechanical model for simulation of the expected performance of the implant under physiological movement (swallowing, sneezing). Laboratory experiments were performed to characterise the tissues present in the trachea, and movement models were obtained from fluoroscopic images of a patient. Results are reported on the planning and biomechanical simulation of two patients that underwent surgery at our hospital.

Intestinal gas and bloating: effect of prokinetic stimulation.

BACKGROUND: It is unknown if abdominal bloating is attributable to excess abdominal gas or improved by a prokinetic agent. AIMS: To assess abdominal gas content in functional abdominal bloating and to ascertain the effect of a prokinetic agent on intestinal gas symptoms in these patients. METHODS: In 20 patients, intra-abdominal gas content and symptoms were quantified before and during treatment with pyridostigmine (30 mg/8 hp. o) in this randomized, placebo-controlled, double-blind study. Daily symptoms were quantified for 5 days before and 10 days during treatment, and abdominal gas volume was quantified by CT imaging before and at the fourth day of treatment. A CT scan was also obtained in 10 healthy subjects. RESULTS: Before treatment, the total volume of intestinal gas was similar in patients (112 +/- 18 mL) and in healthy controls (116 +/- 20 mL). The treatment-induced change in total and regional intestinal gas volume was not significantly different between pyridostigmine (-4 +/- 18 mL; mean +/- SEM) and placebo (0 +/- 15 mL). However, pyridostigmine reduced the severity of bloating from 3.3 +/- 0.3 to 2.6 +/- 0.4 (P < 0.05), whereas placebo did not (3.2 +/- 0.3 vs 3.0 +/- 0.4), although the change did not reach statistical difference across groups. CONCLUSION: In patients complaining of functional bloating, the volume and distribution of intestinal gas, measured on nonselected days, is comparable to asymptomatic subjects. Prokinetic stimulation improves bloating sensation without detectable changes in gas content.

Abdominal accommodation: a coordinated adaptation of the abdominal wall to its content.

BACKGROUND AND AIM: We previously showed that colonic gas infusion increases the girth and modifies the muscular activity of the anterior abdominal wall. We hypothesized that abdominal accommodation to volume loads is an active process instrumented by the coordinated activity of the anterior wall and the diaphragm. METHODS: To increase intraabdominal volume in healthy subjects, a gas was infused into the colon (1.44 L in 1 h) while measuring girth (by tape measure) and electromyography (EMG) activity of the anterior wall (via four pairs of surface electrodes) and the diaphragm (via six ring electrodes over an esophageal tube in the hiatus). After preliminary feasibility studies (N = 12), postural activity (N = 6) and responses to colonic gas loads, both with the trunk erect (N = 8) and in supine position (N = 8), were studied. A morphometric analysis was performed by computed tomography, image analysis (N = 8). RESULTS: In the erect position, anterior wall tone was higher and diaphragmatic tone was lower than in the supine position. With the trunk erect, gas infusion induced diaphragmatic relaxation (by 21 +/- 3%; P < 0.05) and anterior wall contraction (16 +/- 4% EMG increment; P < 0.05). By contrast, in the supine position, it induced diaphragmatic contraction (15 +/- 6%, P < 0.05), while the anterior wall, in the absence of postural tone, showed no change (3 +/- 2%, NS). Gas infusion was associated with girth increase (7.3 +/- 1.0 mm with the trunk erect and 8.6 +/- 1.4 mm in the supine position) and diaphragmatic ascent (17.6 +/- 5.2 mm; P < 0.05). CONCLUSION: The degree of abdominal distension produced by intraabdominal volume increments results from posture-related abdomino-phrenic muscular responses.

Gas distribution within the human gut: effect of meals.

BACKGROUND AND AIMS: Patients frequently complain of gas symptoms precipitated by meals, but the effect of early digestion on intestinal gas content remains unknown. Our aim was to determine the influence of meals on intestinal gas volume and distribution. METHODS: First, we developed a CT image analysis program, based on independent software modules, to measure gas content within the gut. The system was validated in nine healthy subjects by taking helical abdominal CT scans before and after rectal infusion of known volumes of air (100-400 mL). In 15 healthy subjects, intestinal gas distribution was measured in fast and early postcibal CT scans. The postcibal scan was taken 99 +/- 22 minutes after a 597 +/- 57 kcal meal. RESULTS: The volume of gas infused per rectum was detected with an accuracy of 100.4 +/- 3.0%. During fasting, intestinal gas volume was 94 +/- 7 mL (excluding two extreme outliers). After the meal, gas content within the gut increased by 64.7% (up to 149 +/- 21 mL, P < 0.01 vs fast) and the increment occurred in the colon (59 +/- 9 mL precibal vs 121 +/- 20 mL postcibal, P < 0.001), while other gut compartments remained unchanged. CONCLUSION: Ingestion of a meal activated gas metabolism and increased gas content within the gut. The increment occurred early, presumably prior to colonic fermentation of food substrates and was localized in the distal gut, suggesting that gas had a proximal origin and was propelled caudally.